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. Author manuscript; available in PMC: 2010 Jan 25.
Published in final edited form as: Prog Nucleic Acid Res Mol Biol. 2005;80:349–374. doi: 10.1016/S0079-6603(05)80009-1

Table II.

Properties of Ribonuclease A, its Variants, and Onconase®

Ribonuclease kcat/KM (106 M−1s−1) Kd (nM) (kcat/KM)cyto (103 M−1s−1) IC50 (μM) Ref
Wild-type RNase A 43 ± 3 6.7 × 10−5 0.00072 >50 (48–50)
G88R RNase A 14 ± 2 0.57 ± 0.05 2.0 10 ± 1 (48–50)
A4C/G88R/V118C RNase A 2.6 ± 0.2 1.3 ± 0.3 0.84 4.1 ± 0.6 (50)
K41R/G88R RNase A 0.6 ± 0.06 7.5 ± 1.8 1.1 5.2 ± 0.7 (49, 50)
A4C/K41R/G88R/V118C RNase A 0.13 ± 0.03 27 ± 3.7 0.87 7.6 ± 0.9 (50)
K7A/G88R RNase A 8.8 ± 2.6 7.2 ± 0.4 15.8 1.0 ± 0.1 (49)
ONC 0.00035 ± 0.00010 ≥1 × 106 >0.35 0.49 ± 0.065 (49)