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. Author manuscript; available in PMC: 2010 Jan 25.
Published in final edited form as: Nat Biotechnol. 2000 Aug;18(8):847–851. doi: 10.1038/78451

Figure 1.

Figure 1

Genetic selection for dissociative inhibitors of a protein–protein interaction. A. A, Strategy for selecting peptide inhibitors of HIV-1 protease (PR) dimerization. The transcription of reporter genes is turned on when a peptide fused to thioredoxin (Trx) dissociates the hybrid lambda repressor (NcI–PR). Only LacZ+TetR cells survive and form blue colonies in the presence of tetracycline and X-Gal. B, Map of the reporter plasmid (pSH26) and library plasmid used to select for peptide inhibitors of HIV-1 protease dimerization.