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. 2010 Jan 26;5(1):e8891. doi: 10.1371/journal.pone.0008891

Figure 9. Itk mediated signals regulate the expression of the CD4 lineage commitment factor Th-POK.

Figure 9

(A) Quantitative real-time RT-PCR for Th-POK, TOX, Runx3 and GATA3 in sorted transgenic TCRhi DP thymocytes from OT-II and OT-II/Itk−/− mice. Data are corrected for GAPDH expression and expressed as fold over mRNA of the transgenic TCRhi DP derived from OT-II mice, which was set at 1 (n = 3, *p<0.05). The experiment was repeated 2X with the same results. (B) Quantitative real-time RT-PCR for Th-POK, TOX, Runx3 and GATA3 in sorted transgenic TCRhi DP thymocytes from DO11.10 and DO11.10/Itk−/− mice Data are corrected for GAPDH expression and expressed as fold over mRNA of the transgenic TCRhi DP derived from DO11.10 mice, which was set at 1 (n = 3, *p<0.05). The experiment was repeated 2X with the same results. (C) Data used to generate (A) and (B) were rescaled to generate ratios of either OT-II:OTII/Itk−/− or DO11.10:DO11.10/Itk−/− mRNA expression levels for Th-POK, Runx3, GATA3 and TOX.