Figure 11. Itk mediated signals intersect with TCR affinity to regulate Runx3 regulated genes in naïve or conventional CD4⁺ T cells.

Quantitative real-time RT-PCR for Perforin, Granzyme B and Eomesodermin in sorted transgenic TCR^hi CD4SP thymocytes from OT-II and OT-II/Itk^−/− mice Data are corrected for GAPDH expression and expressed as fold over mRNA of the respective OT-II cells, which was set at 1 (n = 3, *p<0.05). Expression of these genes in sorted WT memory phenotype CD8⁺ T cells are shown as a reference.