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. 2010 Jan 26;5(1):e8894. doi: 10.1371/journal.pone.0008894

Figure 1. Alpha-1,2-mannosidase expression is increased in CD25+CD4+ cells after alloantigen re-challenge in vivo.

Figure 1

a) CBA were pre-treated with an anti-CD4 mAb at days -28 and -27 (200 µg YTS 177). At day -27, mice also received an allogeneic blood transfusion (250 µl B10, DST). At days -3 or -1, mice received a DST reboost. CD25+CD4+ and CD25−CD4+ cells were purified from the spleen of these animals at day 0. RNA was extracted from these cells and cDNA was synthesized. (b) Alpha-1,2-mannosidase expression was assessed by Real Time RT-PCR. CD3 expression was measured to normalise the cDNA concentration in each sample. Error bars represent the standard deviation. The data presented are representative of 4 separate experiments.