Figure 6. Ability of Treg^KIF cells to inhibit BM3 T cell priming is impaired.

CBA were pre-treated with an anti-CD4 mAb i.v. at days -28 and -27. At day -27, mice also received specific donor blood transfusion (DST). Treg from these animals were purified at day 0, and incubated for 30 minutes with either PBS or KIF. 5×10⁵ Treg or Treg^KIF were adoptively transferred into CBA-RAG^−/− animals along with 10⁵ CFSE labelled BM3 T cells. One day later (day 0) mice received a B10 skin graft. Lymphoid organs were harvested at day 15. BM3 T cell numbers were analyzed by FACS (cells were gated on Ti98, TCRβ and CD8). n = 4 animals per group. Error bars represent the standard deviation. Data are representative of 3 experiments.