Table 2.
List of important cytochrome P450 enzymes, screening strategies, and possible practical applications
| CYP | Screening strategies | Possible applications in biotechnology, medicine, and bioremediation |
|---|---|---|
| 102 | Fatty acids, alkanes, drugs; H2O2 |
Synthesis of alcohol, indigo and indirubin, and metabolites of propranolol and buspirone; degradation of pollutants, such as PAHs and petroleum components, such as alkanes |
| 101 | Alkanese; H2O2 | Synthesis of alcohols; degradation of petrochemicals, such as alkanes |
| 1A2 | 7-MeQ, 7-MR; NADPH | Synthesis of metabolites of propranolol and warfarin; detoxification of PCBs |
| 1A1/1A2 | Luciferin | Synthesis of metabolites of CYP1A1/1A2's as well as novel drug substrates |
| 2A6 | Indole, 7-MR; NADPH | Syntheiss of indigo compounds |
| 2B1 | 7-EFC; H2O2 | To re-engineer 2B6 or 2B11 for the activation of CPA and IFA |
| 2C8a | C152; CuOOH | Synthesis of metabolites of taxol, chloroquine, and amodiaquine; biomarkers for cancer |
| 2C9a | C152; CuOOH | Synthesis of metabolites of diclofenac, warfarin, and flurbiprofen |
| 2C18a | C152; CuOOH | Activation of CPA and IFA |
| 2C19a | C152; CuOOH | Synthesis of metabolites of diclofenac and warfarin; activation of CPA and IFA |
| 2Cs | Luciferin; NADPH | Synthesis of metabolites of CYP2C's as well as novel drug substrates |
| 3A4 | 7-BQ; CuOOH, NADPH | Synthesis of metabolites of several drugs; activation of IFA |
a
Directed evolution of these P450s are underway. 2Cs: 2C8, 2C9, 2C18, and 2C19.