Figure 5.
Effect of FcR or TLR2/1 activation of peripheral blood monocytes on IL-1β (A) and IL-8 (B) secretion. Percolled monocytes from healthy donors were stimulated overnight with a lipopetide known to stimulate TLR2/1 or plate-bound hIgG in media (open squares), thalidomide R (filled triangles) or thalidomide S (filled circles) enantiomers. IL-1β and IL-8 were measured from cultured supernatant by ELISA. Shown are averages of triplicate cultures +/− SEM. Asterisks denote differences between media and thalidomide-treated cells with p<0.05 calculated by paired Student’s t-test.