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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Infect Dis. 2010 Feb 15;201(4):534–543. doi: 10.1086/650203

Figure 4.

Figure 4

Flagellin induces a variety of cytokines. Mice were treated with metronidazole, neomycin, and vancomycin (MNV) for 7 days. Three doses of flagellin (15μg/day) were administered intraperitoneally to mice beginning on day 5 of MNV treatment. Messenger RNA was extracted from the distal ileum to assess cytokine induction after flagellin treatment using quantitative real-time PCR. IL-12 refers to IL-12p35, and IL-23 refers to IL23p19. Data were pooled from two independent experiments with a total of four mice per group. Levels were normalized to GAPDH and are expressed as mean ± SEM (*p < 0.05, statistical analysis by one-way ANOVA with Bonferroni correction)