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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Infect Dis. 2010 Feb 15;201(4):534–543. doi: 10.1086/650203

Figure 5.

Figure 5

IL-22 is required for flagellin-mediated RegIIIγ expression. Wild-type (WT) and IL-22-deficient (IL-22-KO) or IL-10Rβ-deficient (IL-10Rβ-KO) mice were administered metronidazole, neomycin, and vancomycin (MNV) for 7 days. Mice received 15μg flagellin intraperitoneally on day 6 and 7 of MNV treatment. Tissue from the duodenum (A, C) and ileum (B, D - F) were collected for mRNA and protein extraction. A, B, and E, Quantitative PCR was used to evaluate RegIIIγ mRNA transcript expression. Levels were normalized to GAPDH and expressed as mean ± SEM (*p<0.05, statistical analysis by one-way ANOVA with Bonferroni correction). C, D, and B, Western blot analysis with RegIIIγ-specific anti-serum was used to assess RegIIIγ protein levels. Each lane is a representative sample from a mouse from the indicated group. A - D, Data were pooled from two independent experiments (n=8–10).