Fig. 3.
Effect of the MnCl2 and MgCl2 concentration on PmHS2 activity. Enzymatic activity of purified PmHS2 (55–60 μg/ml) was assayed in the standard polymerization buffer described in the Materials and methods section with an equimolar mixture of divalent metal ions (MnCl2 and MgCl2) ranging from 0 to 16 mM. One hundred percent activity corresponds to the amount of substrate converted after 24 h of incubation at 30 °C in presence of 4 mM of MnCl2 and 4 mM of MgCl2. The data represent the average of three independent experiments ± standard deviation (SD)