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Western blot analysis of histone 3 lysine 9 acetylation in liver nuclear extracts after 3 and 12 hours of ethanol bolus. Note there were no significant changes in the liver of ethanol fed rats compared with the controls. The acetylation of histone 3 lysine 18 (H3K18) also showed no change in the acute ethanol fed model. There was a significant increase of H3K18 acetylation at the peaks in the chronic ethanol fed model (mean ± SEM, n = 3 to 4).
