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. Author manuscript; available in PMC: 2010 Jan 26.
Published in final edited form as: Dev Dyn. 2006 Jan;235(1):29–37. doi: 10.1002/dvdy.20559

Fig. 3.

Fig. 3

Effects of PTK 787 and FK506 on hemoglobinized blood levels and circulation. Embryos treated with PTK 787 and FK506 as described in Fig. 2 were stained with o-dianisidine for circulating hemoglobinized red blood cells(Iuchi and Yamamoto, 1983) in the yolk sac and heart (30). (a) control embryos at 48 hpf. (b) PTK787 treatment for 4h had no effect on oxygenated blood levels and circulation. (c) 12 h and (d) 24 h treatment with PTK 787 resulted in decreased blood in yolk-sac and heart, especially in embryos treated for 24h (d). FK506 did not affect oxygenated blood levels or circulation, even after 24 h exposure. (4h, e; 12h, f; 24h, g).