Figure 3.
The presence of BQCA has no effect on the ACh concentration-response curve at any other mAChR subtype. a–d, No shift in the ACh CRC was observed in the presence of 3 μm BQCA in CHO K1 cells stably expressing M2-Gqi5 (a), M3 (b), M4-Gqi5 (c), or M5 (d) receptors. Calcium mobilization was measured in response to increasing concentrations of ACh following preincubation of cells with either vehicle (■) or 3 μm BQCA (▴), as described in Materials and Methods. Data represent the mean ± SEM of three independent experiments.