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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cancer Res. 2009 Oct 20;69(21):8499–8506. doi: 10.1158/0008-5472.CAN-09-2213

Figure 3.

Figure 3

Depletion of mutant K-ras and mRNA expression of HIF-1α Total RNA was extracted from DKs-5 and DKO-3 cells (DKs) or HK2-10 and HKe-3 (HKs) cultured in normoxia (A), or from DLD-1 and HCT116 cells treated with control siRNA or K-ras siRNA (B). Relative levels of HIF-1α mRNA were determined by qRT-PCR and data were normalized to the levels of 18S rRNA. All reactions were performed in triplicate and data represent the mean ± SD for 3 different cDNA samples. C, DKs-5 and DKO-3 cells were incubated in hypoxia for the indicated times. Relative levels of HIF-1α mRNA were determined by qRT-PCR and data were normalized to the levels of 18S rRNA at each time point. Graph shows the mean ± SD relative to time 0 in each cell line for 2 different cDNA samples.