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. Author manuscript; available in PMC: 2010 Jan 26.
Published in final edited form as: Physiol Genomics. 2007 Jul 24;31(2):352–356. doi: 10.1152/physiolgenomics.00276.2006

Table 1.

Hepatotoxicants used in this study

Compound Doses Vehicle
Bromobenzene L: 25 mg/kg, M: 75 mg/kg, H: 250 mg/kg Corn oil
1,2-Dichlorobenzene L: 15 mg/kg, M: 150 mg/kg, H: 1500 mg/kg Corn oil
1,4-Dichlorobenzene L: 15 mg/kg, M: 150 mg/kg, H: 1500 mg/kg Corn oil
Diquat L: 5 mg/kg, M-L: 10 mg/kg, M-H: 20 mg/kg,
H: 25 mg/kg		 PBS, pH 7.4
Galactosamine L: 25 mg/kg, M: 100 mg/kg, H: 400 mg/kg PBS, pH 7.4
Monocrotaline L: 10 mg/kg, M: 50 mg/kg, H: 300 mg/kg PBS, pH 7.4
N-Nitrosomorpholine L: 10 mg/kg, M: 50 mg/kg, H: 300 mg/kg PBS, pH 7.4
Thioacetamide L: 15 mg/kg, M: 50 mg/kg, H: 150 mg/kg PBS, pH 7.4

All compounds were administered via a single oral dose (5 mL/kg BW) to male Fischer rats (approximately 12 to 14 weeks of age), except for diquat and galactosamine, which were administered i.p (n = 4 animals per treatment group). The dose levels for each hepatotoxicant (except for 1,4-dichlorobenzene, a non-toxic isomer of 1,2-dichlorobenzene) were determined to give the following information: lowest dose (L) – little or no toxicity, moderate dose (M) – mild to moderate toxicity, highest dose (H) – moderate to marked toxicity. For diquat, M-L stands for moderate-low and M-H stands for moderate-high. All animals were fasted 12-18 hours prior to dosing. PBS = phosphate buffered saline.