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. 2010 Jan 26;118(Pt 8):519–526. doi: 10.1042/CS20090422

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© 2010 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Left panels from the top: FcγRIIa (green)/IL-8 (red) x60. Big left panel: co-localization (yellow) of FcγRIIa (green) and IL-8 (red). Nuclei are stained blue. Right panels from the top: FcγRIIa (green)/IL-8 (red) ×60. Big right panel: co-localization (yellow) of FcγRIIa (green) and IL-8 (red). Nuclei are stained blue. Acquisition settings for the images were UV/567/647, and specific parameters for the fluorophores were Cy3 (red) excitation at 567 nm and emission at 600 nm, Alexa 647 (pseudo-colour green) excitation at 647 nm and emission at 700 nm. The sections were evaluated using a PerkinElmer Ultra VIEW LCI confocal imaging system with a Nikon TE2000-S fluorescence microscope and a PlanApo'60 immersion oil objective. Ultra VIEW Imaging Suite software (version 5.5.0.4) was used for image processing.