Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1993 Oct;61(10):4286–4292. doi: 10.1128/iai.61.10.4286-4292.1993

Reproduction of porcine proliferative enteropathy with pure cultures of ileal symbiont intracellularis.

S McOrist 1, S Jasni 1, R A Mackie 1, N MacIntyre 1, N Neef 1, G H Lawson 1
PMCID: PMC281156  PMID: 8406817

Abstract

Porcine proliferative enteropathy is consistently associated with the presence of intracellular curved bacteria in epithelial cells in affected portions of intestine. Two strains of these intracellular bacteria were cultured in a cell culture system with rat enterocytes (IEC-18) and passaged several times and used as oral inocula for 14 gnotobiotic and 8 conventional pigs. DNA and immunological studies had identified these bacteria as belonging to a new taxon, Ileal symbiont (IS) intracellularis. Conventional pigs dosed with approximately 3.7 x 10(6) of these organisms passaged six times in cell culture developed severe lesions of proliferative enteropathy in the ileum. Other conventional pigs dosed with a lower titer or with organisms passaged 13 times developed moderate and minor lesions, respectively. All gnotobiotic pigs dosed with organisms failed to develop lesions. Control pigs, eight conventional and two gnotobiotic, dosed with diluent, uninfected cell material or left undosed failed to develop lesions also. Reisolation of IS intracellularis and demonstration of the organism in mucosal and fecal samples only occurred in conventional pigs dosed with organisms. Gnotobiotic pigs lacking a normal intestinal flora have not been shown to be colonized by the organism. Seroconversion to IS intracellularis or mucosal infiltration by inflammatory cells was not observed in experimentally affected pigs, confirming the weak immune response characteristic of the natural disease. These results support the identification of IS intracellularis as an etiological agent of proliferative enteropathy in pigs.

Full text

PDF
4291

Images in this article

4288Image
on p.4288
4289Image
on p.4289
4289Image
on p.4289
4290Image
on p.4290
4290Image
on p.4290
4290Image
on p.4290

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Biester H. E., Schwarte L. H. Intestinal Adenoma in Swine. Am J Pathol. 1931 Mar;7(2):175–185.6. [PMC free article] [PubMed] [Google Scholar]
  3. Boosinger T. R., Thacker H. L., Armstrong C. H. Campylobacter sputorum subsp mucosalis and Campylobacter hyointestinalis infections in the intestine of gnotobiotic pigs. Am J Vet Res. 1985 Oct;46(10):2152–2156. [PubMed] [Google Scholar]
  4. Garcia F. U., Wojta J., Broadley K. N., Davidson J. M., Hoover R. L. Bartonella bacilliformis stimulates endothelial cells in vitro and is angiogenic in vivo. Am J Pathol. 1990 May;136(5):1125–1135. [PMC free article] [PubMed] [Google Scholar]
  5. Gebhart C. J., Barns S. M., McOrist S., Lin G. F., Lawson G. H. Ileal symbiont intracellularis, an obligate intracellular bacterium of porcine intestines showing a relationship to Desulfovibrio species. Int J Syst Bacteriol. 1993 Jul;43(3):533–538. doi: 10.1099/00207713-43-3-533. [DOI] [PubMed] [Google Scholar]
  6. Gebhart C. J., Lin G. F., McOrist S. M., Lawson G. H., Murtaugh M. P. Cloned DNA probes specific for the intracellular Campylobacter-like organism of porcine proliferative enteritis. J Clin Microbiol. 1991 May;29(5):1011–1015. doi: 10.1128/jcm.29.5.1011-1015.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gebhart C. J., Ward G. E., Chang K., Kurtz H. J. Campylobacter hyointestinalis (new species) isolated from swine with lesions of proliferative ileitis. Am J Vet Res. 1983 Mar;44(3):361–367. [PubMed] [Google Scholar]
  8. Lawson G. H., McOrist S., Jasni S., Mackie R. A. Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro. J Clin Microbiol. 1993 May;31(5):1136–1142. doi: 10.1128/jcm.31.5.1136-1142.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lawson G. H., McOrist S., Rowland A. C., McCartney E., Roberts L. Serological diagnosis of the porcine proliferative enteropathies: implications for aetiology and epidemiology. Vet Rec. 1988 Jun 4;122(23):554–557. doi: 10.1136/vr.122.23.554. [DOI] [PubMed] [Google Scholar]
  10. Lawson G. H., Rowland A. C. Intestinal adenomatosis in the pig: a bacteriological study. Res Vet Sci. 1974 Nov;17(3):331–336. [PubMed] [Google Scholar]
  11. Love R. J., Love D. N., Edwards M. J. Proliferative haemorrhagic enteropathy in pigs. Vet Rec. 1977 Jan 22;100(4):65–68. doi: 10.1136/vr.100.4.65. [DOI] [PubMed] [Google Scholar]
  12. Mapother M. E., Joens L. A., Glock R. D. Experimental reproduction of porcine proliferative enteritis. Vet Rec. 1987 Dec 5;121(23):533–536. [PubMed] [Google Scholar]
  13. McCartney E., Lawson G. H., Rowland A. C. Behaviour of Campylobacter sputorum subspecies mucosalis in gnotobiotic pigs. Res Vet Sci. 1984 May;36(3):290–297. [PubMed] [Google Scholar]
  14. McOrist S., Boid R., Lawson G. H., McConnell I. Monoclonal antibodies to intracellular campylobacter-like organisms of the porcine proliferative enteropathies. Vet Rec. 1987 Oct 31;121(18):421–422. doi: 10.1136/vr.121.18.421. [DOI] [PubMed] [Google Scholar]
  15. McOrist S., Lawson G. H. Reproduction of proliferative enteritis in gnotobiotic pigs. Res Vet Sci. 1989 Jan;46(1):27–33. [PubMed] [Google Scholar]
  16. McOrist S., Lawson G. H., Roy D. J., Boid R. DNA analysis of intracellular Campylobacter-like organisms associated with the porcine proliferative enteropathies: novel organism proposed. FEMS Microbiol Lett. 1990 Jun 1;57(3):189–193. doi: 10.1016/0378-1097(90)90063-v. [DOI] [PubMed] [Google Scholar]
  17. McOrist S., MacIntyre N., Stokes C. R., Lawson G. H. Immunocytological responses in porcine proliferative enteropathies. Infect Immun. 1992 Oct;60(10):4184–4191. doi: 10.1128/iai.60.10.4184-4191.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moulder J. W. Comparative biology of intracellular parasitism. Microbiol Rev. 1985 Sep;49(3):298–337. doi: 10.1128/mr.49.3.298-337.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roberts L., Rowland A. C., Lawson G. H. Experimental reproduction of porcine intestinal adenomatosis and necrotic enteritis. Vet Rec. 1977 Jan 1;100(1):12–13. doi: 10.1136/vr.100.1.12. [DOI] [PubMed] [Google Scholar]
  20. Rowland A. C., Lawson G. H. Intestinal adenomatosis in the pig: immunofluorescent and electron microscopic studies. Res Vet Sci. 1974 Nov;17(3):323–330. [PubMed] [Google Scholar]
  21. Stills H. F., Jr Isolation of an intracellular bacterium from hamsters (Mesocricetus auratus) with proliferative ileitis and reproduction of the disease with a pure culture. Infect Immun. 1991 Sep;59(9):3227–3236. doi: 10.1128/iai.59.9.3227-3236.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Whipp S. C., Robinson I. M., Harris D. L., Glock R. D., Matthews P. J., Alexander T. J. Pathogenic synergism between Treponema hyodysenteriae and other selected anaerobes in gnotobiotic pigs. Infect Immun. 1979 Dec;26(3):1042–1047. doi: 10.1128/iai.26.3.1042-1047.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES