Table III.

Antibody Isotyping Reagents Specificity Testing

Coat Reagent	OD values
	Anti-lgG-HRP	Anti-lgG1-HRP	Anti-lgG2-HRP	Anti-lgG3-HRP	Anti-lgG4-HRP	Anti-lgM-HRP	Anti-lgE-HRP	Biotin anti-lgG4 and avidin-D-HRP
lgG1	2.694	3.264	0.125	0.080	0.079	0.073	0.069	0.141
lgG2	3.532	0.077	1.927	0.082	0.069	0.072	0.066	0.115
lgG3	0.501	0.070	0.063	2.561	0.070	0.073	0.066	0.079
lgG4	3.191	0.154	0.084	0.104	3.864	0.084	0.073	3.039
gM	0.101	0.079	0.069	0.072	0.071	3.893	0.065	0.076
lgE	0.098	0.076	0.067	0.090	0.067	0.073	2.313	Not tested

The reagent specificity of the isotyping detectors including mouse anti-human IgG1-HRP, mouse anti-human IgG2-HRP, mouse anti-human IgG3-HRP, mouse anti-human IgG4-HRP, mouse anti-human IgG-HRP, mouse anti-human IgM-HRP, mouse anti-human IgE-HRP, and biotin mouse anti-human IgG4 were confirmed by coating the microtiter plate with various purified antibody isotypes (IgG1, IgG2, IgG3, IgG4, IgM, and IgE) at 1.0 μg/ml and detecting with the corresponding isotyping detectors, respectively. Avidin-D-HRP was used in conjunction with biotin-mouse anti-human IgG4