Table VIII.
Confirmation of the Specificity of the IgG4 Type Anti-CHO-HCP Antibody in Normal Human Serum (HS) Samples—Uncoated Plate Specificity Testing
Human serum | Mean OD | Reported as | |
---|---|---|---|
Coated | Uncoated | ||
HS-8 | 2.517 | 0.121 | Positive/Specific |
HS-41 | 0.573 | 0.598 | Positive/Non-specific |
HS-43 | 0.477 | 0.144 | Positive/Specific |
HS-6a | 0.127 | 0.147 | Negative |
HS-23a | 0.080 | 0.071 | Negative |
HS-63a | 0.119 | 0.124 | Negative |
HS-B13a | 0.094 | 0.084 | Negative |
HS-B14a | 0.079 | 0.081 | Negative |
Samples were analyzed on the CHO-HCP-coated and uncoated plates using the biotin mouse anti-human IgG4 antibody detector in conjunction with avidin-D-HRP
aTested negative for anti-CHD-HCP reactivity