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. 2010 Feb;16(2):430–441. doi: 10.1261/rna.1808510

FIGURE 4.

FIGURE 4.

Protein expression analysis from human cells transfected with siRNA complementary to the VEGF gene promoter or GRB2 mRNA. (A) VEGF expression in HeLa cells transfected with siGRB2 or control siRNA. VEGF expression from cells transfected with each siRNA is normalized to “Mock” transfected cells and presented as the percentage mean ± SD from three independent experiments, with three replicate samples per experiment; (*) p < 0.05. (B) Western blot analysis for the detection and quantitation of GRB2 protein extracted from HeLa cells treated with siVFp(−992) or control siRNA. A representative blot is shown with samples loaded per lane, as follows: (lane 1) Mock transfected cells; (lane 2) siVFp(−992); (lane 3) siVFp(−992)m1; and (lane 4) siGRB2. The intensity of each band for GRB2 was quantified by densitometry and normalized to that for β-actin. GRB2 protein levels from HeLa cells transfected with each siRNA are shown as a ratio relative to Mock transfected cells and presented as the percentage mean ± range from two independent experiments, with two replicate samples per experiment.