FIGURE 1.

Primer design strategy for PCR-based detection of DNA contamination in total RNA samples. The possible presence of DNA contamination is analyzed by PCR using special primers for the HNRNPA2B1 gene. The forward and reverse primers are located in two different neighboring exons amplifying a 109-bp fragment from cDNA. In the presence of genomic DNA, it gives rise to an additional amplicon of 184 bp in size due to the additional intron.