Fig 2.

γ-Globin expression in wild type and β-thalassaemia intermedia mice. Peripheral blood was collected at 10–20 weeks of age from wild type and Hbb^th–3/+ individuals containing one or two copies of the γ^lo transgene alleles (γ^lo/− and γ^lo/γ^lo respectively) or two copies of the γ^hi transgene allele (γ^hi/γ^hi). Samples were analysed for the amount of γ-globin RNA (as a percentage of total mouse α-globin, open bars) by RNase protection, and for the amount of γ-globin protein (in the form of hybrid mouse/human HbF as a percentage of total Hb, grey bars) by high-performance liquid chromatography. Results are presented as mean ± SD for a total of five to 12 individuals for each group for the RNA analysis and seven to 29 individuals for each group for the protein analysis. *P < 0.001 vs. RNA; P < 0.001 vs. wild type.