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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Proteome Res. 2009 Jul;8(7):3746–3751. doi: 10.1021/pr801122b

Figure 1.

Figure 1

Elution peaks (plotted as intensity vs elution time) for the transition 418.2 → 860.4 acquired at seven different collision energies. The second decimal place of the product m/z values listed in the figure codes for the collision energy used, with 860.41 corresponding to the lowest CE tested (default CE −6 V) and 860.47 corresponding to the highest CE tested (CE +6 V). For this particular transition, the plot clearly shows that the signal is maximal at 2 V higher than the default CE (magenta line) and decreases monotonically for both higher and lower CEs.