Skip to main content
. 2010 Jan 27;5(1):e8917. doi: 10.1371/journal.pone.0008917

Figure 1. Construction of a FRET reporter for glutamine and in vitro properties of the purified protein.

Figure 1

(A) Schematic design of the reporter. (B,C) FRET reporter with mutation D157N. (B) Kd app = 6.9 mM Gln, dynamic range r max−r min = 2.21 (n = 3). (D) Specificity to all proteinogenic amino acids. Ratio change upon addition of 3 mM (black bars) or 10 mM (white bars) of the amino acids given in tree letter code (n = 3). (D,E) FRET reporter with mutations D157N and T70A. (D) Kd app = 18.8 mM Gln, dynamic range r max−r min = 2.31 (n = 3). (E) Specificity and ratio change upon addition of 3 mM (black bar) or 10 mM (white bar) of the amino acids given in tree letter code (n = 3).