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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Biochemistry. 2010 Feb 2;49(4):653–655. doi: 10.1021/bi902155t

Figure 2.

Figure 2

Assigned 1H-15N TROSY-HSQC NMR spectrum of uniformly-2H,13C,15N-labeled KCNE3 in isotropic bicelles (buffer: 250 mM imidazole, 2 mM DTT, pH 6.5). The sample contained 22% (w/v) bicelles with DHPC:DMPG = 3:1 (q = 0.33). NMR experiments were carried out at 40 °C on a Bruker 800 MHz spectrometer equipped with a cryogenic probe as described in the Supporting Information. Backbone resonance assignments are tabulated in BioMagResBank entry 16621.