Table III.
Protein | k’CO(µM−1 s−1) | kCO(s−1) | KCO(µM−1) |
---|---|---|---|
Nitrobindin (Fe2+) A | 0.23 | 0.050 | 4.5 |
SWMb WT(Fe2+) B | 0.53 | 0.019 | 28 |
SWMb H64Q(Fe2+) B | 0.94 | 0.012 | 78 |
SWMb H64L (Fe2+) B | 26 | 0.024 | 1,100 |
Soybean Lba(Fe2+) C | 17 | 0.0078 | 2,200 |
Nitrophorin 4 pH 8.0 D | 7.9 | n.d. | n.d. |
Protein | k’NO(µM−1 s−1) | kNO(s−1) | KNO(µM–1) |
Nitrobindin (Fe2+) A | 81 | ~0.08 | ~1,000 |
SWMb WT (Fe2+) B | 22 | 0.000098 | 220,000 |
SWMb H64Q (Fe2+) B | 43 | 0.00011 | 370,000 |
SWMb H64L (Fe2+) B | 190 | 0.00013 | 1,500,000 |
Soybean Lb a (Fe2+) C | 170 | 0.00002 | 9,000,000 |
Nitrobindin (Fe3+) A | 1.2 | 73 | 0.016 |
metSWMb WT (Fe3+) B | 0.080 | 12 | 0.0067 |
SW metMb H64Q (Fe3+) B | 11 | 59 | 0.19 |
SW metMb H64L (Fe3+) B | 44 | 80 | 0.55 |
Soybean metLba(Fe3+) E | 0.14 | 3.0 | 0.047 |
Nitrophorin 1 pH 8.0 F | 1.5 | 4.3 | 0.34 |
Nitrophorin 2 pH 8.0 F | 33 | 32 | 1.0 |
Nitrophorin 3 pH 8.0 F | 6.7 | 30 | 0.22 |
Nitrophorin 4 pH 8.0 F | 2.5 | 15 | 0.17 |
Rate constants that were determined in this study were measured in 100 mM potassium phosphate buffer at pH 7 containing EDTA (1 mM) at 20 °C.
The rates were taken from Rohlfs et al. [35], Eich et al. [76], Thorsteinsson et al. [55], Foley [36].
Taken from Hargrove et al. [75].
Rates taken from Maes et al. [48]
Taken from Herold et al. [77]