Fig. 4.

PAI-1 expression in primary cultured VSMCs of SHP^-/- mice. RT-PCR analysis of PAI-1 and SHP mRNA expression in primary cultured VSMCs from WT (SHP^+/+) and SHP-knockout (SHP^-/-) mice. Cells were incubated in medium containing 0.5% FBS for 24 h, and were then treated with angiotensin II (Ang II, 10 nM) for 3 h before harvesting. Data represent the means ± SEM. ^*P < 0.05 was as compared to WT VSMC (SHP^+/+) without Ang II, ^**P < 0.05 was as compared to KO VSMC (SHP^-/-) without Ang II, and ^#P < 0.05 was as compared to WT VSMC (SHP^+/+) with Ang II.