Figure 3.

Math5 and Ngn3 overexpression lead to reduction in PC proliferation (A–C). GFP⁺ cells in retinal sections of E12.5 retinal explants that were electroporated with plasmids expressing GFP (A), Math5 plus GFP (B), and Ngn3 plus GFP (C). (A′–C′) Quantification of GFP⁺ cells shown in A–C using the automated spot-detection (white circles) algorithm (IMARIS, Bitplane) with a diameter threshold of 5 μm and pixel intensity of 21. (A″–C″) Quantification of the number of GFP⁺ cells identified in A′–C′ that are also EdU⁺. (D) Bar graph of data in A′–C″ representing the number of GFP⁺ cells that were EdU⁺ in control (green), Math5 plus GFP (red), and Ngn3 (yellow). The P-values shown here were obtained by performing the Student's t-test. (E) A model showing how AS can regulate the number of PCs by regulating the production of noncoding RNA versus protein-coding RNA.