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. 2010 Feb 1;24(3):256–264. doi: 10.1101/gad.1878510

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Copyright © 2010 by Cold Spring Harbor Laboratory Press

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Figure 5. — Gene expression in the hypothalamus and pituitary gland of N^WT and N^KO mice. (A) N^WT and N^KO mice were fed a chow diet (ND) or a HFD (16 wk) and then fasted overnight. The expression of AgRP, leptin receptor, somatostatin, gonadotrophin-releasing hormone (GnRH), PMCH, POMC, CART, and GH-releasing hormone (GHRH) mRNA in the hypothalamus was measured by quantitative RT–PCR. The data were normalized to the expression of Gapdh mRNA in each sample. The data are presented as the mean ± SD (n = 6). Statistically significant differences between N^WT and N^KO mice are indicated ([*] P < 0.05). (B) The expression of TSHβ, GH, and POMC mRNA in the pituitary gland was measured by quantitative RT–PCR. The data were normalized to the expression of Gapdh mRNA in each sample. The data are presented as the mean ± SD (n = 6). Statistically significant differences between N^WT and N^KO mice are indicated ([*] P < 0.05; [**] P < 0.01).