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. 2010 Feb 1;24(3):301–311. doi: 10.1101/gad.1864510

Figure 3.

Figure 3.

The hmcns mutation is caused by an A nucleotide insertion in the Zfp191 gene. (A) Schematic of chromosome 18 (Chr. 18) showing hmcns localized between the markers D18Mit117 and D18Mit14. (B) With the addition of markers between D18Mit117 and D18Mit14, the critical region of the mutation was mapped to the region between the SNP rs13483237 and the SSLP marker D18Mit34. Black boxes and white boxes represent alleles derived from C57BL/6J (normal) and C3H/HeJ (mutation background) mice, respectively. (C, top) The area defined by fine mapping contains 29 genes. (Bottom) Zfp191 is a gene consisting of four exons. The arrow indicates the location of the adenine insertion in the gene. (D) Chromatogram showing the A insertion in the mutant sequence. (E) RT–PCR analysis of Zfp191 expression, which revealed a transcript in several tissues in addition to the CNS.