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. 2010 Feb 1;24(3):301–311. doi: 10.1101/gad.1864510

Figure 6.

Figure 6.

Conditional knockout of Zfp191 in oligodendrocytes results in hypomyelination. (A) IHC of wild-type and Zfp191 conditional mutant corpus callosum using anti-MBP showed reduced myelin in the oligodendrocyte-specific Zfp191 knockout. Bar, 50 μM. Samples were from P21 mice. (B) qPCR of Mbp, Plp, and Cgt mRNA found that message expression was reduced for all three genes in Zfp191 conditional mutant brains. Diagonally striped boxes represent wild-type brains, and gray boxes represent mutants. Error bars represent STD. All expression data were significantly different (P < 0.05). Samples were from P21 mice. (C) Western blotting found that the levels of MAG and MBP protein are severely reduced in Zfp191 conditional mutant brains. Samples were from P21 mice. (D) Observation by TEM revealed that many axons in the mutant spinal cord are unmyelinated (P21). Bar, 1 nm.