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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Curr Biol. 2010 Jan 20:nihpa168171. doi: 10.1016/j.cub.2009.12.033

Figure 3.

Figure 3

Deleting the CEN3 locus decreases cell cycle delay caused by the DNA DSB at MAT. A) An HO cut site was inserted to the left of CEN3. A 2 kb fragment from the left of the HO cutsite was inserted to the right of CEN3. Gal-HO induction creates DSBs at MAT and near CEN3. CEN3 is deleted as the nearby DSB is repaired by SSA between flanking homologous DNA segments. B) CEN3 is deleted within 3 h of HO induction, as seen in a southern blot probed with the sequences (black) flanking CEN3. C) Deleting CEN3 shortens the DNA DSB-dependent cell cycle arrest in WT cells but not in mad2Δ cells. An equivalent SSA event occurring at the URA3 locus does not affect cell cycle arrest. Deleting CEN3 does not affect arrest when the DSB is made on another chromosome. Deleting CEN3 shortens the checkpoint in a chk1Δ strain albeit not as much as deleting MAD2.