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. 2009 Nov 26;13(6):R188. doi: 10.1186/cc8182

Table 1.

Primers and endonucleases for genotyping of IL-10 promoter single nucleotide polymorphisms

SNPs Primers PCR conditions Restriction endonucleases
-1082A/G F:ACACAAATCCAAGACAACACTACTAAGGCTTCCTTGGGA 3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 56°C, 40 seconds at 72°C, then 10 minutes at 72°C XagI
R:GTGATCAAACAGAGGCACAGACAT
-819T/C F:CACTCTAAGGCTTCCTTGGGA 3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 56°C, 40 seconds at 72°C, then 10 minutes at 72°C Hin1α
R:CCTACCGTCTCTATTTTATAGTGAGCAAACTGAGGCACAGACAT
-592 A/C F:AGCTGAAGAGGTGGAAACATGTG 3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 63°C, 40 seconds at 72°C, then 10 minutes at 72°C Rsa I
R:TGGGTTCTCATTCGCGTGTT