-1082A/G |
F:ACACAAATCCAAGACAACACTACTAAGGCTTCCTTGGGA |
3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 56°C, 40 seconds at 72°C, then 10 minutes at 72°C |
XagI |
|
R:GTGATCAAACAGAGGCACAGACAT |
|
|
-819T/C |
F:CACTCTAAGGCTTCCTTGGGA |
3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 56°C, 40 seconds at 72°C, then 10 minutes at 72°C |
Hin1α |
|
R:CCTACCGTCTCTATTTTATAGTGAGCAAACTGAGGCACAGACAT |
|
|
-592 A/C |
F:AGCTGAAGAGGTGGAAACATGTG |
3 minutes at 94°C followed by 35 cycles of 40 seconds at 94°C, 45 seconds at 63°C, 40 seconds at 72°C, then 10 minutes at 72°C |
Rsa I |
|
R:TGGGTTCTCATTCGCGTGTT |
|
|