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. 2009 Oct 28;29(43):13578–13588. doi: 10.1523/JNEUROSCI.4390-09.2009

Figure 7.

Figure 7.

Lentivirus-mediated delivery of beclin 1 to α-syn tg mice reduces α-syn accumulation. Immunocytochemical and confocal analysis 3 months after injection. A, B, Levels of beclin 1 immunoreactivity in α-syn tg mice (line D) that were injected with LV-control. C, D, Beclin 1 immunoreactivity in α-syn tg mice treated with LV-Beclin 1. Arrows (A, C) indicate injection tract in the neocortex. Low-power images of the brain (A, C) were taken with a 5× objective, and higher-magnification images (B, D) were taken with a 40× objective. E, Immunoblot analysis of brain tissue from the injection site to confirm expression of beclin 1. F–N, Confocal analysis of sections double immunolabeled with antibodies against α-syn (red) and beclin 1 (green). F–H, α-syn tg mice that were injected with LV-control. I–N, Colocalization of α-syn and beclin 1 in discrete granular structures in tg mice injected with LV-Beclin 1. Samples were further stained with DAPI to visualize nuclei (n) (blue). Arrows indicate areas of beclin 1 and α-syn colocalization. Scale bar, 5 μm.