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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Fertil Steril. 2008 Feb 20;91(2):616–621. doi: 10.1016/j.fertnstert.2007.11.059

FIGURE 3. Western blot of nuclear NF-κB.

FIGURE 3

Nuclear extracts from normal peritoneal and adhesion fibroblasts before and after hypoxia (2% O2) were fractionated with SDS-PAGE. Membrane was probed with anti-NF-κB antibody. A: normal fibroblasts (Lane 1–3), and adhesion fibroblasts (Lane 4–6); B: results were analyzed by NIH image J 3.0. *P<0.0001 compared to normal peritoneal fibroblasts cultured under normoxic conditions. ** P<0.0001 compared to adhesion fibroblasts cultured under normoxic conditions. *** P<0.0001 compared to normal fibroblasts cultured under normoxic conditions. NF/AF, normal/adhesion fibroblast. Hyp-24/Hyp-48, normal/adhesion fibroblast exposed to hypoxia for 24h and 48h.