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Effects of Tat on the half-life of cyclin B1. Levels of cyclin B1 were measured by Western blot. Actin was used as the internal loading control. A) CHX was added to the culture of TE671-pCI and TE671-Tat cells. B) TE671-pCI and TE671-Tat cells were exposed to 4 Gy. Cells were treated with 200 ng/ml of CHX and were collected at different times.
