Figure 5.

Effect of Noggin expression on formation of cordlike structures and effect of conditioned cell culture medium on the formation of cords by WT HUVECs. A, C) Conditioned by GFP⁺ (A) or GFP/Nog⁺ HUVECs seeded on the surface of Matrigel (C). B, D) Ability of GFP⁺ (B) and GFP/Nog⁺ HUVECs (D) to form cords was improved by adding 20 ng/ml hrBMP-4. E, F) Formation of cords by GFP⁺ (E) and GFP/Nog⁺ HUVECs (F), detected by using fluorescence microscopy. G) Quantitation of cords using phase-contrast microscopy. Data are presented as means ± sd (n=8). Scale bars= 500 μm.