Figure 1.

Functional characterization and reannotation of the M. tuberculosis oligopeptide (Opp) transport system. A) WT M. tuberculosis and the dpp, PDIM− (fadD26), and opp mutants, including the complemented (Comp) opp mutant, were cultured on solid medium in the absence (left plate, NO) or presence (right plate, YES) of bialaphos at a 5 μg/ml concentration, and incubated at 37°C for 3 wk. B) WT, opp mutant (opp KO), and its complemented strains were plated for single colonies on solid medium and incubated for 7 wk at 37°C. C) Growth curve (CFU/ml) of WT, opp KO, and complemented strains.