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NMDAR subunit levels are not altered in RMOR-knock-in mice after chronic morphine treatment. Protein levels of the NMDAR subunits NR1 (A), NR2A (B), and NR2B (C) were assessed in several mouse brain regions by immunoblot in both WT and RMOR-knock-in mice treated with morphine or saline. Data are expressed as means ± se. Tissue samples were analyzed from ≥3 separate experiments. Thal, thalamus; PAG, periaqueductal gray. *P < 0.05, **P < 0.01, ***P < 0.001 vs. saline group.
