Figure 1.
Comparison of sarcomere structure and force-pCa relation between wt and nebulin−/− psoas muscle fibers from 1-d-old mice. A) Transmission electron microscopy on longitudinal sections of psoas muscle from wt and nebulin−/− mice. Arrow indicates a zone of Z-line widening. Scale bar = 2 μm. B) Force-pCa relation in fiber bundles from wt and nebulin−/− mice. Solid (wt) and dashed (nebulin−/−) lines are Hill sigmoidal equations (Eq. 1) fitted to data. Inset: mean ± se values of the parameters. wt psoas: bundle length, 1.00 mm; sarcomere length, 2.57 μm; CSA, 2300 μm2; T0, 71 kPa; temperature, 13.5°C. nebulin−/− psoas: bundle length, 1.50 mm; sarcomere length, 2.60 μm; CSA, 4300 μm2; T0, 24 kPa; temperature, 13.4°C.