Fig. 2.

Analysis of Ca²⁺ mobilization in phagocytes treated with AG-09/1 and AG-09/4. HL-60-FPR1 and HL-60-FPR2 cells (A and B) or human neutrophils (C and D) were loaded with fluorometric imaging plate reader calcium 3 dye, and Ca²⁺ flux was analyzed, as described under Materials and Methods. Responses were normalized to the response induced by 5 nM fMLF for HL-60-FPR1 cells and neutrophils, or 5 nM WKYMVm for HL-60-FPRL1 cells, which were assigned a value of 100%. D, human neutrophils were treated with 5 μM concentrations of the compounds under investigation (AG-09/1 and AG-09/4), 5 nM fMLF (positive control), or 1% DMSO (negative control), and Ca²⁺ flux was monitored for the indicated times (arrow indicates when treatment was added). The data are from one experiment that is representative of three independent experiments.