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Stimulated cAMP efflux also depends on the background Na+ conductance. Effect of complete replacement of extracellular Na+ with NMDG and TMA on cAMP release in forskolin- (A and C) and GHRH (B and D)-stimulated cells. In our preparation of mixed populations of pituitary cells, approximately 40% of cells express GHRH receptors (somatotrophs). Data shown are representative of three similar experiments.
