Fig. 5.

Modulation of RORα-mediated G6Pase promoter activity and CYP7B1 promoter activity by T0901317 (T1317). 293T cells were cotransfected with pS6 control plasmid or pS6 containing full-length RORα along with G6Pase promoter (A) or CYP7B1 promoter (B) as detailed under Materials and Methods. SRC2 as a coactivator was also cotransfected with G6Pase promoter. Dose-response curve was determined by treating the transfected cells with varying concentrations of T0901317 for 20 h. Luciferase activity was measured, and relative change was determined by normalizing to cells treated with vehicle only. Each data point was performed in eight replicates and is represented as mean ± S.E.M., n = 8.