TABLE 1.
TRH receptor affinity
Either HEK293 or arrestin2/3 knockout MEFs were transfected with WT or 6Q-TRH receptors. Equilibrium binding of [3H]MeTRH was measured, and Kd values were determined by Scatchard analysis. Values shown are the mean ± S.E.M. from three experiments. Data for WT receptor in arrestin-null cells are from Jones and Hinkle (2008).
| Cell Type |
Kd |
|
|---|---|---|
| WT-TRH Receptor | 6Q-TRH Receptor | |
| nM | ||
| HEK293 | 2.2 ± 0.2 | 9.1 ± 0.1 |
| Arrestin2/3KO MEF | ||
| Mock | 20.1 ± 2.6 | 13.8 ± 3.6 |
| +Arrestin3 | 1.4 ± 0.2 | 11.9 ± 1.5 |