FIG. 5.

Effects of anti-TLR2 antibody, anti-TLR4 antibody, and LPS-37 on the ability of FKB-27 to stimulate cytokine production in U937 cells. U937 cells were cultured with anti-TLR2 antibody (10 μg/ml), anti-TLR4 antibody (10 μg/ml), or LPS-37 (1 ng/ml) for 1 h; and then FKB-27 (3 × 10⁵/ml) or LPS-27 (1 ng/ml) was added to stimulate the cells. The cytokine levels in the culture supernatants were determined by ELISA. The data are presented as the means ± standard errors for triplicate samples. A representative result from three independent experiments is shown. *, P < 0.05 compared to the results for the control, as determined by Student's t test.