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Detection of IL-1β-specific bands by immunoblotting. Lysozyme-treated cell extracts (CE), concentrated culture supernatants (S), and 10 ng of purified IL-1β (1B) were applied to SDS-PAGE and immunoblotting. Anti-IL-1β antibody was used at 1:5,000, and HRP-conjugated anti-rabbit IgG was used at 1:50,000. Molecular masses are shown on the left.
