FIG. 3.

Biological activity of recombinant murine IL-1β produced by KJ725. Human IL-8 released from Caco-2 cells was measured after 24 h of stimulation with culture supernatant of KJ725 (empty squares) or LCN (filled squares) or with purified murine IL-1β standard (empty circles). The concentration of total murine IL-1β in the culture supernatant of KJ725 (in MRSEC medium) was determined by ELISA and then adjusted to 100, 50, and 25 pg/ml by dilution in complete RPMI medium. The corresponding volume of culture supernatant of LCN was added to the Caco-2 cell culture as a negative control. The result shown is representative of three separate experiments; the values are the means ± standard deviations (SD) of duplicate samples.