FIG. 2.

Immunoreactivities of recombinant E. chaffeensis Ank200 proteins by Western immunoblotting. Top, SDS-PAGE and total protein staining of purified recombinant Ank200 proteins (N, N-terminal fragment; I, internal fragment; and C, C-terminal fragment). Bottom, corresponding Western immunoblot probed with a patient serum sample (no. SC07). The patient serum sample did not recognize thioredoxin protein, and the normal human serum sample did not recognize these recombinant proteins by Western immunoblotting (data not shown). M, Precision protein standard (Bio-Rad).