FIG. 4.

Schematic of E. chaffeensis TRP47 and immunoreactivities of recombinant TRP47-N proteins by Western immunoblotting. (A) Schematic of TRP47, showing domains, locations of TRs (number of amino acids in parentheses), and the recombinant proteins and synthetic peptides used for epitope mapping. The recombinant proteins and synthetic peptides are shown with black lines and gray lines, respectively, and the solid lines show regions containing an epitope(s). (B) Western immunoblot assay of four recombinant proteins representing TRP47-N probed with three HME patient serum samples (no. O13, O15, and 19).